Journal Article

IMPULSE-2021-00285

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Neutron crystallography for the elucidation of enzyme catalysis

Kono, F. (Corresponding author) ; Tamada, T. (Corresponding author)

2021
Elsevier Amsterdam [u.a.]

Please use a persistent id in citations: doi:10.1016/j.sbi.2021.05.007

Abstract: Hydrogen atoms and hydration water molecules in proteins are indispensable for many biochemical processes, especially enzymatic catalysis. The locations of hydrogen atoms in pro- teins are usually predicted based on X-ray structures, but it is still very difficult to know the ionization states of the catalytic residues, the hydration structure of the protein, and the char- acteristics of hydrogen-bonding interactions. Neutron crystal- lography allows the direct observation of hydrogen atoms that play crucial roles in molecular recognition and the catalytic reactions of enzymes. In this review, we present the current status of neutron crystallography in structural biology and recent neutron structural analyses of three enzymes: ascor- bate peroxidase, the main protease of severe acute respiratory syndrome coronavirus 2, and copper-containing nitrite reductase.

Keyword(s): Health and Life (1st) ; Biology (2nd)

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Contributing Institute(s):

1. BIODIFF (BIODIFF)
2. STRUCTURE (STRUCTURE)

Experiment(s):

1. BIODIFF: Diffractometer for large unit cells (NL1)

Appears in the scientific report 2021

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Database coverage:
; BIOSIS Previews ; BIOSIS Reviews Reports And Meetings ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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