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2021-09-20
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2021-09-20
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2021-09-20
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2021-09-15
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2021-09-15
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2021-09-15
11:00
[IMPULSE-2021-00285] Journal Article
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Neutron crystallography for the elucidation of enzyme catalysis
Hydrogen atoms and hydration water molecules in proteins are indispensable for many biochemical processes, especially enzymatic catalysis. The locations of hydrogen atoms in pro- teins are usually predicted based on X-ray structures, but it is still very difficult to know the ionization states of the catalytic residues, the hydration structure of the protein, and the char- acteristics of hydrogen-bonding interactions. [...]
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2021-09-15
10:04
[IMPULSE-2021-00284] Journal Article
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How a Fragment Draws Attention to Selectivity Discriminating Features between the Related Proteases Trypsin and Thrombin
In the S1 pocket, the serine proteases thrombin and trypsin commonly feature Asp189 and a Ala190Ser and Glu192Gln exchange. Nevertheless, thrombin cleaves peptide chains solely after Arg, and trypsin after Lys and Arg. [...]
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2021-09-14
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2021-09-14
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2021-09-14
11:56

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