Journal Article

IMPULSE-2022-00167

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Porphyrin Binding and Irradiation Promote G-Quadruplex DNA Dimeric Structure

Libera, V. ; Andreeva, E. A. ; Martel, A. ; Thureau, A. ; Longo, M.MLZ* ; Petrillo, C. ; Paciaroni, A. ; Schirò, G. ; Comez, L.

2021
ACS Washington, DC

Please use a persistent id in citations: doi:10.1021/acs.jpclett.1c01840

Abstract: Nucleic acid sequences rich in guanines can organize into noncanonical DNA G-quadruplexes (G4s) of variable size. The design of small molecules stabilizing the structure of G4s is a rapidly growing area for the development of novel anticancer therapeutic strategies and bottom-up nanotechnologies. Among a multitude of binders, porphyrins are very attractive due to their light activation that can make them valuable conformational regulators of G4s. Here, a structure-based strategy, integrating complementary probes, is employed to study the interaction between TMPyP4 porphyrin and a 22-base human telomeric sequence (Tel22) before and after irradiation with blue light. Porphyrin binding is discovered to promote Tel22 dimerization, while light irradiation of the Tel22-TMPyP4 complex controls dimer fraction. Such a change in quaternary structure is found to be strictly correlated with modifications at the secondary structure level, thus providing an unprecedented link between the degree of dimerization and the underlying conformational changes in G4s.

Keyword(s): Polymers, Soft Nano Particles and Proteins (1st) ; Biology (2nd) ; Medicine (2nd) ; Soft Condensed Matter (2nd)

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Contributing Institute(s):

1. BIODIFF (BIODIFF)

Experiment(s):

1. SANS-1: Small angle neutron scattering (NL4a)

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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